BD Pharmingen 7-AAD核酸染料 559925，7-Amino-Actinomycin D (7-AAD)

（7-AAD） 是一種即用型核酸染料，可替代碘化丙啶（PI）用于流式細(xì)胞檢測中非活細(xì)胞的排除。與PI相比，7-AAD的優(yōu)勢在于能與藻紅蛋白（PE）和異硫氰酸熒光素（FITC）標(biāo)記的單克隆抗體聯(lián)用，進(jìn)行雙色分析，且7-AAD、PE和FITC的熒光發(fā)射光譜重疊極小。7-AAD的熒光在遠(yuǎn)紅光光譜范圍內(nèi)檢測（650 nm長通濾光片）。

該試劑作為一種活力探針，基于光散射和FL3通道檢測的7-AAD攝取，用于死細(xì)胞排除方法。無需稀釋，建議用量為每測試（1×10^6個(gè)細(xì)胞）添加5 µl（0.25 µg），分析前孵育10分鐘。

7-Amino-Actinomycin D (7-AAD) is a convenient, ready-to-use nucleic acid dye that can be used in place of propidium iodide (PI) for the exclusion of nonviable cells in flow cytometric assays. The advantage of 7-AAD over PI is the ability to be used in conjunction with phycoerythrin (PE)- and fluorescein isothiocyanate (FITC)-labelled monoclonal antibodies in 2-color analysis, with minimal spectral overlap between 7-AAD, PE and FITC fluorescence emissions. The 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).  This reagent is used as a viability probe for methods of dead cell exclusion, based on light scatter and uptake of 7-AAD as detected in FL3.  This reagent does not require dilution.  Suggested quantity to use:  5 µl (0.25 µg)/test (1x10^6 cells) and incubate for 10 minutes before analysis.

BD Pharmingen 7-AAD核酸染料 559925，7-Amino-Actinomycin D (7-AAD)

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.